The USGS BRD has science and technology centers located throughout the United States. Typically, activities are closely aligned with the diversity of biological resources near each center.

Science center researchers represent a broad mix of scientific disciplines. Areas of expertise include aquatic systems (toxic material and other stressors), botany, ecosystem analysis and modeling, fishery chemistry and management, forest management, genetics, invertebrate zoology, museum curation, nutrition, phenology, statistical design and analysis, taxonomy and systematics, toxicology, urban ecology, and wildlife habitat evaluation.