Sequencing Unusual STR Alleles
Members of the human identity testing community can submit unusual STR alleles to the the Biochemical Science Division at the National Institute of Standards and Technology (NIST) for sequencing. Sequencing is free to the submitting agency .
Some of these unusual STR alleles possess point mutations in prime binding sites leading to null alleles while others are "off-ladder" variants. Sequencing these unusual alleles helps to reveal the molecular basis for their variation.
Samples requires at least 10 ng of genomic DNA. To submit a sample or if you have questions about submitting a sample for STR allele sequencing, contact either Margaret Kline (301-975-3134; margaret.kline@nist.gov) or John Butler (301-975-4049; john.butler@nist.gov).
- Margaret Kline talk at 57th Annual Meeting of the American Academy of Forensic Sciences (PDF), New Orleans, LA, February 25, 2005, "Some Interesting Point Mutations and Deletions Found Through STR Allele Sequencing"
- Margaret Kline talk at Bode East Coast Advanced DNA Technology Workshop (PDF), Duck Key, FL, May 24, 2005, "Finding Point Mutations, Deletions and New Alleles Through STR Allele Sequencing"
Content on this page provided by the Biochemical Science Division,National Institute of Standards and Technology, STRBase Web site. Partial support for the design and maintenance of that website is provided by the Office of Justice Programs' National Institute of Justice through the NIST Office of Law Enforcement Standards.
