Wayne F. Patton PerkinElmer Life and Analytical Sciences Office: 781-863-5514 FAX: E-mail: wayne.patton@perkinelmer.com Job Title: Director of Biochemistry Ph.D. in Molecular and Cellular Biology from the University of Massachusetts

Speaker: Wayne F. Patton, PerkinElmer Life and Analytical Sciences, 940 Winter Street, Waltham, MA 02451

Topic: Quantitative Protein Expression and Phosphorylation Analysis: Tools for Proteomics Analysis

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, May 8, 2007, at 2:00 PM

Abstract: Mass tag kits for multiplexed analysis of proteins by tandem mass spectormetry contain up to ten reactive isobaric peptides that can be covalently attached to either cysteine or lysine residues in proteins (kits are available with 2-, 4-, 7- and 10-plex capability). Proteins in up to ten samples can be processed at the same time to provide large gains in productivity relative to existing quantitative methods. Furthermore, the abiliaty to process 7 - 10 samples simultaneously allows for the first time pharmacological applications such as kinetic and dose responses based on protein expression from a single experiment. An equally important benefit is obtained by labeling at the protein level, such that standard proteomic enrichment/separation methods (i.e. affinty enrichment, gel separations) can be performed for improved complexity reduction, resulting in the ability to focus more on low abundance proteins.