Standard operating procedure with chloroform in the Monolayer Lab and the
X-Ray Lab (B36-B37, B39)
Chloroform will be mainly used to
prepare solutions of organic materials (lipids, fatty-acids, polymers, proteins
etc.). The solutions will be prepared
and manipulated under a ventilated hood. The solutions will be labeled and kept
in a refrigerator, and waste materials will be collected in waste bottles that
will be appropriately labeled and disposed of according to the regulations at
the Ames Laboratory.
Small amounts (generally 50 - 100
micro-liters) of solution will be spread from a syringe on liquid surfaces
(water solutions) at the sample position of the diffractometer in the
beamline. A film of the organic
material will be formed after the evaporation of the chloroform. To minimize the evaporation of chloroform
into air, the sample holder will be sealed immediately after spreading the
film. The frequency of spreading films
during a run is about 1-2 a day.
The
experimenter will use 6 or 8 mil thick Nitrile gloves (NOT latex and NOT
polyethylene) for splash protection, provided that as soon as exposure of the
glove to any liquid chloroform occurs, work is immediately stopped and the
contaminated glove removed and replaced with a clean fresh glove. Contaminated gloves, papers, and other
items will be collected and dried in the hood.
Heavy Viton gloves will be required if you wish to clean the
trough (in the hood only) with chloroform.
No cleaning of the Langmuir trough or any item be performed outside the
ventilated hood.
Waste chloroform will be
collected in a labeled and dated waste bottle (according to AL rules) and will
be cleared from the lab by ESH 30 days
after initial date of the bottle.
In case of a small spill (below
30 ml) use absorbing paper to collect and the clean the area immediately, place
the papers in the ventilated hood and mark the area on the floor. Then clean the floor with water and soap. In case of a spill of over 30 ml mark call
ESH representative and mark the area to prevent anybody from getting close to
the contaminated area.
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